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This study was designed to investigate the correlation between autophagy and polarization of macrophages in atherosclerosis (AS) plaque in arteriosclerosis obliterans amputees. Femoral artery specimens from arteriosclerosis obliterans amputees were performed hematoxylin and eosin (HE) staining, oil red O and immunofluorescence staining to observe the morphology of atherosclerotic plaque, phenotype of macrophages and autophagy in plaque; using real-time quantitative RT-PCR technology to detect the mRNA level of M1 and M2 type markers in arterial tissue; to analyze polarized signal pathway and autophagy protein levels in macrophages by Western blotting. Arterial specimens staining showed obvious lipid deposition and obvious infiltration of amount of foam cells and inflammatory cells. Macrophages were mainly expression M1 type in percentage in fibrous plaque. Although both M1 and M2 macrophages were upregulated in atheromatous plaque, the increase was dominant in M2 type in percentage. The level of autophagy was significantly higher in the atheromatous plaque than that of fibrous plaque. The expression of tumor necrosis factor- α (TNF-α), monocyte chemotactic protein-1 (MCP-1), inducible nitric oxide synthase (iNOS), interleukin-6 (IL-6) and interleukin-12 (IL-12) mRNA was significantly higher in fibrous plaque than that of atheromatous plaque (P < 0.01 or 0.05), and arginase-1 (Arg-1), transforming growth factor-β (TGF-β), CD163 and interleukin-10 (IL-10) mRNA was significantly lower than that in atheromatous plaque (P < 0.01). The levels of p-STAT1 and NF-κB were significantly increased in fibrous plaque (P < 0.01), while p-STAT6 expression was significantly increased in atheromatous plaque (P < 0.01). The level of LC3-II was significantly higher in atheromatous plaque than that in fibrous plaque (P < 0.01). Macrophages in early atherosclerotic plaque were induced to M1 type through p-STAT1/NF-κB pathway and expressed moderate levels of autophagy; while macrophages in advanced plaques were induced to polarization of M2 type through p-STAT6 pathway. M2 macrophages expressed a higher level of autophagy than M1 macrophages.
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Objective To observe the microstructural changes in lesions of alopecia areata (AA) with dermoscopy and to evaluate their correlation with clinicopathological manifestations. Methods The area of alopecia of 62 patients with AA and 44 patients with other types of hair loss were observed by using a noncontact polarized dermoscope (Dermlite, USA). Clinical data on and laboratory findings from these patients were collected. Pathological examination was carried out with scalp biopsy specimens from the alopecia area of 15 AA patients. Results Characteristic dermoscopic signs of AA included yellow dots, black dots, broken hairs, exclamation mark hairs, short vellus hair and newly-grown short hairs. Among these signs, yellow dots showed the highest prevalence (83.9%). Exclamation mark hairs, black dots and broken hairs were rather specific signs for AA, and the prevalence of the three signs was positively correlated with disease activity and positivity rate of hair-pull test. A positive correlation was also noted between the prevalence of elevated thyroid peroxidase antibody levels and positivity rate of hair-pull test (r = 0.269, P ＜ 0.05 ) as well as prevalence of broken hairs (r = 0.445, P ＜ 0.05), and between the prevalence of yellow dots and that of keratinous plug in follicular orifice. There was a negative correlation between the prevalence of newly-grown short hairs and perifollicular mast cell infiltration and between the prevalence of black dots and the anagen/catagen ratio. Conclusions Yellow dots can serve as a preliminary screening marker for AA. Exclamation mark hairs, black dots and broken hairs are highly sensitive for the confirmation of diagnosis of AA, and often predict progressive AA.Dermoscopic signs are well correlated to the histopathology features of AA, and may be useful for the evaluation of disease severity and guidance on the treatment of AA.
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Objective To investigate the influence of individual psychological intervention on quality of life of HIV infection / AIDS patients.Methods 50 cases diagnosed as HIV infection / AIDS were selected,randomly divided into the control group and the observation group with 25 cases in each group.The control group received routine treatment and care,while for the observation group,the psychological health and quality of life were measured with SCL-90,SF-36 scales,then personalized psychological intervention was implemented after analyzing the influencing factors.Three months after discharge,the assessment test was carried out using SCL-90 and SF-36 scales,then compared the quality of mental health and life situation of the two groups.Results Mental health and quality of life between the observation group and the control group before intervention was not statistically significant.After intervention,the comparison is statistically significant Conclusions Individualized psychological intervention can improve patients quality of life.
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Objective To explore the effect of dietary intervention on treatment effect and life quality of young patients with hepatitis B. Methods 106 cases of hepatitis B patients were randomly divided into the intervention group and the control group, each group with 53 cases. The patients in the control group were given medication treatment and conventional diet, while the intervention group was given medication treatment plus dietary intervention. The therapeutic effect and quality of life were evaluated between the two groups 30 days after the intervention. Results 29 cases in the intervention group were cured (54.72%),while only 16 cases in the control group (30.19%) were cured. The total score of life quality in the intervention group was (69.72 ± 8.42), and (55.71 ± 8.31) in the control group. Significant difference existed in subsequent dimensionality: emotional role, bodily function,bodily pain, vitality, social function, mental health and general health between the two groups. Conclusions Clinical efficacy can be improved and quality of life can be enhanced through dietary intervention in patients with hepatitis B.
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Objective To explore the impact of psychological intervention on response and defense style of AIDS patients.Methods 88 AIDS patients were selected and investigated using medical response (MCMQ) and defense style (DSQ) survey,the scores before and after psychological intervention were compared.Results Mental health problems included fear,afraid,pessimism and worry,the response style included face,avoid,yield.Scores showed significant difference before and after intervention among patients choosing immature-type,mature-type and cover-up defense styles.Conclusions Psychological intervention can promote rehabilitation and health of AIDS patients as well as improve their life quality and prevent disease relapse.
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Objective To confirm clinical curative effect and pharmacodynamics mechanism of Clean Lung Conquer Acne Decoction in treating lung-heat type acne,as well as its effect on androgen(andrusol). Methods 80 cases of acne were divided into two groups randomly. Treatment group(50 cases) took orally Clean Lung Conquer Acne Decoction,control group(30 cases) took orally Yiqing capsule,8 weeks was a course of treatment. Clinical therapeutic effect and serum andrusol were observed. Results To lung-heat type acne patient,skin damage effective of treatment group compared with control group,P